## ----echo=FALSE, results="hide", warning=FALSE--------------------------------
suppressPackageStartupMessages({
    library(trackViewer)
    library(rtracklayer)
    library(TxDb.Hsapiens.UCSC.hg19.knownGene)
    library(org.Hs.eg.db)
    library(VariantAnnotation)
  library(httr)
})
knitr::opts_chunk$set(warning=FALSE, message=FALSE)

## ----lolliplot1, fig.width=6, fig.height=3------------------------------------
library(trackViewer)
SNP <- c(10, 12, 1400, 1402)
sample.gr <- GRanges("chr1", IRanges(SNP, width=1, names=paste0("snp", SNP)))
features <- GRanges("chr1", IRanges(c(1, 501, 1001), 
                                    width=c(120, 400, 405),
                                    names=paste0("block", 1:3)))
lolliplot(sample.gr, features)
## More SNPs
SNP <- c(10, 100, 105, 108, 400, 410, 420, 600, 700, 805, 840, 1400, 1402)
sample.gr <- GRanges("chr1", IRanges(SNP, width=1, names=paste0("snp", SNP)))
lolliplot(sample.gr, features)
## Define the range
lolliplot(sample.gr, unname(features), ranges = GRanges("chr1", IRanges(104, 109)))

## ----lolliplot2, fig.width=6, fig.height=3------------------------------------
features$fill <- c("#FF8833", "#51C6E6", "#DFA32D")
lolliplot(sample.gr, features)

## ----lolliplot3, fig.width=6, fig.height=3------------------------------------
sample.gr$color <- sample.int(6, length(SNP), replace=TRUE)
sample.gr$border <- sample(c("gray80", "gray30"), length(SNP), replace=TRUE)
sample.gr$alpha <- sample(100:255, length(SNP), replace = TRUE)/255
lolliplot(sample.gr, features)

## ----lolliplot.index, fig.width=6, fig.height=3-------------------------------
sample.gr$node.label <- as.character(seq_along(sample.gr))
sample.gr$node.label.col <- 
  ifelse(sample.gr$alpha>0.5 | sample.gr$color==1, "white", "black")
sample.gr$node.label.cex <- sample.int(3, length(sample.gr), replace = TRUE)/2
lolliplot(sample.gr, features)
sample.gr$node.label.cex <- 1 ## change it back for pretty showcase.

## ----lolliplot4, fig.width=6, fig.height=3------------------------------------
features$height <- c(0.02, 0.05, 0.08)
lolliplot(sample.gr, features)
## Specifying the height and its unit
features$height <- list(unit(1/16, "inches"),
                        unit(3, "mm"),
                        unit(12, "points"))
lolliplot(sample.gr, features)

## ----lolliplot.mul.features, fig.width=6, fig.height=3------------------------
features.mul <- rep(features, 2)
features.mul$height[4:6] <- list(unit(1/8, "inches"),
                                 unit(0.5, "lines"),
                                 unit(.2, "char"))
features.mul$fill <- c("#FF8833", "#F9712A", "#DFA32D", 
                       "#51C6E6", "#009DDA", "#4B9CDF")
end(features.mul)[5] <- end(features.mul[5])+50
features.mul$featureLayerID <- 
    paste("tx", rep(1:2, each=length(features)), sep="_")
names(features.mul) <- 
    paste(features.mul$featureLayerID, 
          rep(1:length(features), 2), sep="_")
lolliplot(sample.gr, features.mul)
## One name per transcript
names(features.mul) <- features.mul$featureLayerID
lolliplot(sample.gr, features.mul)

## ----lolliplot4.1, fig.width=6, fig.height=3.5--------------------------------
#Note: the score value is an integer less than 10
sample.gr$score <- sample.int(5, length(sample.gr), replace = TRUE)
lolliplot(sample.gr, features)
##Remove y-axis
lolliplot(sample.gr, features, yaxis=FALSE)

## ----lolliplot4.20, fig.width=6, fig.height=4.5-------------------------------
#Try a score value greater than 10
sample.gr$score <- sample.int(15, length(sample.gr), replace=TRUE)
sample.gr$node.label <- as.character(sample.gr$score)
lolliplot(sample.gr, features)

## ----lolliplot4.21, fig.width=6, fig.height=5---------------------------------
#increase the cutoff value of style switch.
lolliplot(sample.gr, features, lollipop_style_switch_limit=15)

## ----lolliplot4.22, fig.width=6, fig.height=4.5-------------------------------
#Try a float numeric score
sample.gr$score <- runif(length(sample.gr))*10
sample.gr$node.label <- as.character(round(sample.gr$score, digits = 1))
lolliplot(sample.gr, features)
# Score should not be smaller than 1
# remove the alpha for following samples
sample.gr$alpha <- NULL

## ----lolliplot.xaxis, fig.width=6, fig.height=4.5-----------------------------
xaxis <- c(1, 200, 400, 701, 1000, 1200, 1402) ## define the position
lolliplot(sample.gr, features, xaxis=xaxis)
names(xaxis) <- xaxis # define the labels
names(xaxis)[4] <- "center" 
lolliplot(sample.gr, features, xaxis=xaxis)

## ----lolliplot.yaxis, fig.width=6, fig.height=4.5-----------------------------
#yaxis <- c(0, 5) ## define the position
#lolliplot(sample.gr, features, yaxis=yaxis)
yaxis <- c(0, 5, 10, 15) ## define the position
names(yaxis) <- yaxis # define the labels
names(yaxis)[3] <- "y-axis" 
lolliplot(sample.gr, features, yaxis=yaxis)

## ----lolliplot.jitter, fig.width=6, fig.height=4.5----------------------------
sample.gr$dashline.col <- sample.gr$color
lolliplot(sample.gr, features, jitter="label")

## ----lolliplot.legend, fig.width=6, fig.height=4.5----------------------------
legend <- 1:6 ## legend fill color
names(legend) <- paste0("legend", letters[1:6]) ## legend labels
lolliplot(sample.gr, features, legend=legend)
## use list to define more attributes. see ?grid::gpar to get more details.
legend <- list(labels=paste0("legend", LETTERS[1:6]), 
               col=palette()[6:1], 
               fill=palette()[legend])
lolliplot(sample.gr, features, legend=legend)
## if you have multiple tracks, please try to set the legend by list.
## see more examples in the section [Plot multiple samples](#plot-multiple-samples)
legendList <- list(legend)
lolliplot(sample.gr, features, legend=legendList)

# from version 1.21.8, users can also try to set legend 
# as a column name in the metadata of GRanges.
sample.gr.newlegend <- sample.gr
sample.gr.newlegend$legend <- LETTERS[sample.gr$color]
lolliplot(sample.gr.newlegend, features, legend="legend")
# from version 1.41.6, users can set the legend position to right
lolliplot(sample.gr, features, legend=legend,
          legendPosition = list(position='right',
                                width=unit(1, 'inch')))
## use ncol or nrow to control the legend layout.
legendList[[1]]$ncol <- 2 # if legend is not a list of list, use legend$ncol <- 2
lolliplot(sample.gr, features, legend=legendList, legendPosition = 'right')

## ----lolliplot.labels.control, fig.width=6, fig.height=4----------------------
sample.gr.rot <- sample.gr
sample.gr.rot$label.parameter.rot <- 45
lolliplot(sample.gr.rot, features, legend=legend)
sample.gr.rot$label.parameter.rot <- 60
sample.gr.rot$label.parameter.col <- "brown"
## change the label text into user-defined names other than names of the sample.gr
sample.gr.rot$label.parameter.label <- names(sample.gr)
random_ids <- sample(seq_along(sample.gr), 5)
sample.gr.rot$label.parameter.label[random_ids] <- 
  paste("new label", random_ids)
random_ids <- sample(seq_along(sample.gr), 2)
sample.gr.rot$label.parameter.label[random_ids] <- NA ## remove some labels
lolliplot(sample.gr.rot, features, legend=legend)
## try different colors
sample.gr.rot$label.parameter.col <- sample.int(7,
                                                length(sample.gr),
                                                replace = TRUE)
sample.gr.rot$label.parameter.draw <- TRUE
sample.gr.rot$label.parameter.draw[[1]] <- FALSE ## another method to remove the first label
lolliplot(sample.gr.rot, features, legend=legend)

## ----lolliplot.labels.ctl.one.by.one, fig.width=6, fig.height=4---------------
label.parameter.gp.brown <- gpar(col="brown")
label.parameter.gp.blue <- gpar(col="blue")
label.parameter.gp.red <- gpar(col="red")
sample.gr$label.parameter.gp <- sample(list(label.parameter.gp.blue,
                                            label.parameter.gp.brown,
                                            label.parameter.gp.red),
                                       length(sample.gr), replace = TRUE)
lolliplot(sample.gr, features)

## ----lolliplot.labels.on.feature, fig.width=6, fig.height=4-------------------
lolliplot(sample.gr, features, label_on_feature=TRUE)

## ----lolliplot.xlab.ylab.title, fig.width=6, fig.height=5.2-------------------
lolliplot(sample.gr.rot, features, legend=legend, ylab="y label here")
grid.text("label of x-axis here", x=.5, y=.01, just="bottom")
grid.text("title here", x=.5, y=.98, just="top", 
          gp=gpar(cex=1.5, fontface="bold"))

## ----lolliplot.motiflogo, fig.width=6, fig.height=5.2-------------------------
library(motifStack)
pcms<-readPCM(file.path(find.package("motifStack"), "extdata"),"pcm$")
sample.gr.rot$label.parameter.pfm <- pcms[sample(seq_along(pcms),
                                                 length(sample.gr.rot),
                                                 replace = TRUE)]
lolliplot(sample.gr.rot, features, legend=legend)

## ----lolliplotShape, fig.width=6, fig.height=4.5------------------------------
## shape must be "circle", "square", "diamond", "triangle_point_up", or "triangle_point_down"
available.shapes <- c("circle", "square", "diamond", 
                      "triangle_point_up", "triangle_point_down")
sample.gr$shape <- sample(available.shapes, size = length(sample.gr), replace = TRUE)
sample.gr$legend <- paste0("legend", as.numeric(factor(sample.gr$shape)))
lolliplot(sample.gr, features, type="circle", legend = "legend")
sample.gr.mul.shape <- sample.gr
sample.gr.mul.shape$score <- ceiling(sample.gr.mul.shape$score)
sample.gr.mul.shape$shape <- lapply(sample.gr.mul.shape$score, function(s){
  sample(available.shapes, size = s, replace = TRUE)
})
sample.gr.mul.shape$color <- lapply(sample.gr.mul.shape$score, function(s){
  sample.int(7, size = s, replace = TRUE)
})
lolliplot(sample.gr.mul.shape, features, type="circle",
          lollipop_style_switch_limit = max(sample.gr.mul.shape$score))

## ----lolliplot5, fig.width=6, fig.height=4.5----------------------------------
lolliplot(sample.gr, features, type="pin")
sample.gr$color <- lapply(sample.gr$color, function(.ele) c(.ele, sample.int(6, 1)))
sample.gr$border <- sample.int(6, length(SNP), replace=TRUE)
lolliplot(sample.gr, features, type="pin")

## ----lolliplotFlag, fig.width=6, fig.height=4---------------------------------
sample.gr.flag <- sample.gr
sample.gr.flag$label <- names(sample.gr) ## move the names to metadata:label
names(sample.gr.flag) <- NULL
#lolliplot(sample.gr.flag, features, 
#          ranges=GRanges("chr1", IRanges(0, 1600)), ## use ranges to leave more space on the right margin.
#          type="flag")
## change the flag rotation angle
sample.gr.flag$node.label.rot <- 15
sample.gr.flag$node.label.rot[c(2, 5)] <- c(60, -15)
sample.gr.flag$label[7] <- "I have a long name"
sample.gr.flag$node.label.cex <- 1
sample.gr.flag$node.label.cex[4] <- 2
lolliplot(sample.gr.flag, features, 
          ranges=GRanges("chr1", IRanges(0, 1600)),## use ranges to leave more space on the right margin. 
          type="flag")

## ----lolliplot6, fig.width=6, fig.height=3------------------------------------
sample.gr$score <- NULL ## must be removed, because pie will consider all the numeric columns except column "color", "fill", "alpha", "shape", "lwd", "id" and "id.col".
sample.gr$label <- NULL
sample.gr$node.label.col <- NULL
x <- sample.int(100, length(SNP))
sample.gr$value1 <- x
sample.gr$value2 <- 100 - x # for pie plot, 2 value columns are required.
## the length of the color should be no less than that of value1 or value2
sample.gr$color <- rep(list(c("#87CEFA", '#98CE31')), length(SNP))
sample.gr$border <- "gray30"
lolliplot(sample.gr, features, type="pie")

## ----lolliplot7, fig.width=6, fig.height=5.5----------------------------------
SNP2 <- sample(4000:8000, 30)
x2 <- sample.int(100, length(SNP2), replace=TRUE)
sample2.gr <- GRanges("chr3", IRanges(SNP2, width=1, names=paste0("snp", SNP2)), 
             value1=x2, value2=100-x2)
sample2.gr$color <- rep(list(c('#DB7575', '#FFD700')), length(SNP2))
sample2.gr$border <- "gray30"

features2 <- GRanges("chr3", IRanges(c(5001, 5801, 7001), 
                                    width=c(500, 500, 405),
                                    names=paste0("block", 4:6)),
                    fill=c("orange", "gray30", "lightblue"),
                    height=unit(c(0.5, 0.3, 0.8), "cm"))
legends <- list(list(labels=c("WT", "MUT"), fill=c("#87CEFA", '#98CE31')), 
                list(labels=c("WT", "MUT"), fill=c('#DB7575', '#FFD700')))
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features, y=features2), 
          type="pie", legend=legends)

## ----lolliplot.multiple.type, fig.width=6, fig.height=7.5---------------------
sample2.gr$score <- sample2.gr$value1 ## The circle layout needs the score column 
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features, y=features2), 
          type=c("pie", "circle"), legend=legends)

## ----lolliplot.pie.stack, fig.width=6, fig.height=5---------------------------
rand.id <- sample.int(length(sample.gr), 3*length(sample.gr), replace=TRUE)
rand.id <- sort(rand.id)
sample.gr.mul.patient <- sample.gr[rand.id]
## pie.stack require metadata "stack.factor", and the metadata can not be 
## stack.factor.order or stack.factor.first
len.max <- max(table(rand.id))
stack.factors <- paste0("patient", formatC(1:len.max, 
                                           width=nchar(as.character(len.max)), 
                                           flag="0"))
sample.gr.mul.patient$stack.factor <- 
    unlist(lapply(table(rand.id), sample, x=stack.factors))
sample.gr.mul.patient$value1 <- 
    sample.int(100, length(sample.gr.mul.patient), replace=TRUE)
sample.gr.mul.patient$value2 <- 100 - sample.gr.mul.patient$value1
patient.color.set <- as.list(as.data.frame(rbind(rainbow(length(stack.factors)), 
                                                 "#FFFFFFFF"), 
                                           stringsAsFactors=FALSE))
names(patient.color.set) <- stack.factors
sample.gr.mul.patient$color <- 
    patient.color.set[sample.gr.mul.patient$stack.factor]
legend <- list(labels=stack.factors, col="gray80", 
               fill=sapply(patient.color.set, `[`, 1))
## remove one mutation label
sample.gr.mul.patient$label.parameter.draw <- TRUE
sample.gr.mul.patient$label.parameter.draw[
  names(sample.gr.mul.patient)==
    sample(unique(names(sample.gr.mul.patient)), 1)] <-
  FALSE
lolliplot(sample.gr.mul.patient, features, type="pie.stack", 
          legend=legend, dashline.col="gray")

## ----lolliplot.caterpillar, fig.width=6, fig.height=4-------------------------
sample.gr$SNPsideID <- sample(c("top", "bottom"), 
                               length(sample.gr),
                               replace=TRUE)
lolliplot(sample.gr, features, type="pie", 
          legend=legends[[1]])

## ----lolliplot.caterpillar2, fig.width=6, fig.height=12-----------------------
## Two layers
sample2.gr$SNPsideID <- "top"
idx <- sample.int(length(sample2.gr), 15)
sample2.gr$SNPsideID[idx] <- "bottom"
sample2.gr$color[idx] <- '#FFD700'
lolliplot(list(A=sample.gr, B=sample2.gr), 
          list(x=features.mul, y=features2), 
          type=c("pie", "circle"), legend=legends)

## ----ProteinsAPI, fig.width=6, fig.height=3, eval=FALSE, echo=TRUE------------
#  library(httr) # load library to get data from REST API
#  APIurl <- "https://www.ebi.ac.uk/proteins/api/" # base URL of the API
#  taxid <- "9606" # human tax ID
#  gene <- "TP53" # target gene
#  orgDB <- "org.Hs.eg.db" # org database to get the uniprot accession id
#  eid <- mget("TP53", get(sub(".db", "SYMBOL2EG", orgDB)))[[1]]
#  chr <- mget(eid, get(sub(".db", "CHR", orgDB)))[[1]]
#  accession <- unlist(lapply(eid, function(.ele){
#    mget(.ele, get(sub(".db", "UNIPROT", orgDB)))
#  }))
#  stopifnot(length(accession)<=20) # max number of accession is 20
#  
#  tryCatch({ ## in case the internet connection does not work
#    featureURL <- paste0(APIurl,
#                         "features?offset=0&size=-1&reviewed=true",
#                         "&types=DNA_BIND%2CMOTIF%2CDOMAIN",
#                         "&taxid=", taxid,
#                         "&accession=", paste(accession, collapse = "%2C")
#    )
#    response <- GET(featureURL)
#    if(!http_error(response)){
#      content <- httr::content(response)
#      content <- content[[1]]
#      acc <- content$accession
#      sequence <- content$sequence
#      gr <- GRanges(chr, IRanges(1, nchar(sequence)))
#      domains <- do.call(rbind, content$features)
#      domains <- GRanges(chr, IRanges(as.numeric(domains[, "begin"]),
#                                       as.numeric(domains[, "end"]),
#                                       names = domains[, "description"]))
#      names(domains)[1] <- "DNA_BIND" ## this is hard coding.
#      domains$fill <- 1+seq_along(domains)
#      domains$height <- 0.04
#      ## GET variations. This part can be replaced by user-defined data.
#      variationURL <- paste0(APIurl,
#                             "variation?offset=0&size=-1",
#                             "&sourcetype=uniprot&dbtype=dbSNP",
#                             "&taxid=", taxid,
#                             "&accession=", acc)
#      response <- GET(variationURL)
#      if(!http_error(response)){
#        content <- httr::content(response)
#        content <- content[[1]]
#        keep <- sapply(content$features, function(.ele) length(.ele$evidences)>2 && # filter the data by at least 2 evidences
#                         !grepl("Unclassified", .ele$clinicalSignificances)) # filter the data by classified clinical significances.
#        nkeep <- c("wildType", "alternativeSequence", "begin", "end",
#                   "somaticStatus", "consequenceType", "score")
#        content$features <- lapply(content$features[keep], function(.ele){
#          .ele$score <- length(.ele$evidences)
#          unlist(.ele[nkeep])
#        })
#        variation <- do.call(rbind, content$features)
#        variation <-
#          GRanges(chr,
#                  IRanges(as.numeric(variation[, "begin"]),
#                          width = 1,
#                          names = paste0(variation[, "wildType"],
#                                         variation[, "begin"],
#                                         variation[, "alternativeSequence"])),
#                  score = as.numeric(variation[, "score"]),
#                  color = as.numeric(factor(variation[, "consequenceType"]))+1)
#        variation$label.parameter.gp <- gpar(cex=.5)
#        lolliplot(variation, domains, ranges = gr, ylab = "# evidences", yaxis = FALSE)
#      }else{
#        message("Can not get variations. http error")
#      }
#    }else{
#      message("Can not get features. http error")
#    }
#  },error=function(e){
#    message(e)
#  },warning=function(w){
#    message(w)
#  },interrupt=function(i){
#    message(i)
#  })

## ----VCF, fig.width=6, fig.height=5-------------------------------------------
library(VariantAnnotation)
library(TxDb.Hsapiens.UCSC.hg19.knownGene)
library(org.Hs.eg.db)
fl <- system.file("extdata", "chr22.vcf.gz", package="VariantAnnotation")
gr <- GRanges("22", IRanges(50968014, 50970514, names="TYMP"))
if(.Platform$OS.type!="windows"){# This line is for avoiding error from VariantAnnotation in the windows platform, which will be removed when VariantAnnotation's issue gets fixed.
tab <- TabixFile(fl)
vcf <- readVcf(fl, "hg19", param=gr)
mutation.frequency <- rowRanges(vcf)
mcols(mutation.frequency) <- cbind(mcols(mutation.frequency), 
                                   VariantAnnotation::info(vcf))
mutation.frequency$border <- "gray30"
mutation.frequency$color <- 
    ifelse(grepl("^rs", names(mutation.frequency)), "lightcyan", "lavender")
## Plot Global Allele Frequency based on AC/AN
mutation.frequency$score <- mutation.frequency$AF*100
seqlevelsStyle(mutation.frequency) <- "UCSC"
if(!grepl("chr", seqlevels(mutation.frequency)[1])){
  seqlevels(mutation.frequency) <- 
    paste0("chr", seqlevels(mutation.frequency))
}
}
seqlevelsStyle(gr) <- "UCSC" 
trs <- geneModelFromTxdb(TxDb.Hsapiens.UCSC.hg19.knownGene,
                         org.Hs.eg.db,
                         gr=gr)
features <- c(range(trs[[1]]$dat), range(trs[[5]]$dat))
names(features) <- c(trs[[1]]$name, trs[[5]]$name)
features$fill <- c("lightblue", "mistyrose")
features$height <- c(.02, .04)
if(.Platform$OS.type!="windows"){
lolliplot(mutation.frequency, features, ranges=gr)
}

## ----methylation, eval=FALSE, echo=TRUE---------------------------------------
#  library(rtracklayer)
#  session <- browserSession()
#  query <- ucscTableQuery(session,
#                          table="wgEncodeHaibMethylRrbs",
#                          range=GRangesForUCSCGenome("hg19",
#                                                     seqnames(gr),
#                                                     ranges(gr)))
#  tableName(query) <- tableNames(query)[1]
#  methy <- track(query)
#  methy <- GRanges(methy)

## ----methylation.hide, echo=FALSE---------------------------------------------
methy <- import(system.file("extdata", "methy.bed", package="trackViewer"), "BED")

## ----fig.width=6,fig.height=4-------------------------------------------------
lolliplot(methy, features, ranges=gr, type="pin")

## ----fig.width=6,fig.height=2.5-----------------------------------------------
methy$lwd <- .5
lolliplot(methy, features, ranges=gr, type="pin", cex=.5)
#lolliplot(methy, features, ranges=gr, type="circle", cex=.5)
methy$score2 <- max(methy$score) - methy$score
lolliplot(methy, features, ranges=gr, type="pie", cex=.5)
## We can change it one by one
methy$cex <- runif(length(methy))
lolliplot(methy, features, ranges=gr, type="pin")
#lolliplot(methy, features, ranges=gr, type="circle")

## ----fig.width=6,fig.height=2.5-----------------------------------------------
methy$cex <- 1
lolliplot(methy, features, ranges=gr, rescale = TRUE)
## by set percentage for features and non-features segments
xaxis <- c(50968014, 50968514, 50968710, 50968838, 50970514)
rescale <- c(.3, .4, .3)
lolliplot(methy, features, ranges=gr, type="pin",
          rescale = rescale, xaxis = xaxis)
## by set data.frame to rescale
rescale <- data.frame(
  from.start = c(50968014, 50968515, 50968838), 
  from.end   = c(50968514, 50968837, 50970514),
  to.start   = c(50968014, 50968838, 50969501), 
  to.end     = c(50968837, 50969500, 50970514)
)
lolliplot(methy, features, ranges=gr, type="pin",
          rescale = rescale, xaxis = xaxis)

## ----fig.width=6,fig.height=2.5-----------------------------------------------
lolliplot(methy, features, ranges=gr, rescale = "exon")
# exon region occupy 99% of the plot region.
lolliplot(methy, features, ranges=gr, rescale = "exon_99")
lolliplot(methy, features, ranges=gr, rescale = "intron")

## ----fig.width=9,fig.height=8-------------------------------------------------
grSplited <- tile(gr, n=2)
lolliplot(methy, features, ranges=grSplited, type="pin")

## ----fig.width=8,fig.height=4-------------------------------------------------
gene <- geneTrack(get("HSPA8", org.Hs.egSYMBOL2EG), TxDb.Hsapiens.UCSC.hg19.knownGene)[[1]]
SNPs <- GRanges("chr11", IRanges(sample(122929275:122930122, size = 20), width = 1), strand="-")
SNPs$score <- sample.int(5, length(SNPs), replace = TRUE)
SNPs$color <- sample.int(6, length(SNPs), replace=TRUE)
SNPs$border <- "gray80"
SNPs$feature.height = .1
gene$dat2 <- SNPs
extdata <- system.file("extdata", package="trackViewer",
                       mustWork=TRUE)
repA <- importScore(file.path(extdata, "cpsf160.repA_-.wig"),
                    file.path(extdata, "cpsf160.repA_+.wig"),
                    format="WIG")
fox2 <- importScore(file.path(extdata, "fox2.bed"), format="BED",
                    ranges=GRanges("chr11", IRanges(122830799, 123116707)))
optSty <- optimizeStyle(trackList(repA, fox2, gene), theme="col")
trackList <- optSty$tracks
viewerStyle <- optSty$style
gr <- GRanges("chr11", IRanges(122929275, 122930122))
setTrackStyleParam(trackList[[3]], "ylabgp", list(cex=.8))
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)
## lollipopData track
SNPs2 <- GRanges("chr11", IRanges(sample(122929275:122930122, size = 30), width = 1), strand="-")
SNPs2 <- c(SNPs2, promoters(gene$dat, upstream = 0, downstream = 1))
SNPs2$score <- sample.int(3, length(SNPs2), replace = TRUE)
SNPs2$color <- sample.int(6, length(SNPs2), replace=TRUE)
SNPs2$border <- "gray30"
SNPs2$feature.height = .1
lollipopData <- new("track", dat=SNPs, dat2=SNPs2, type="lollipopData")
gene <- geneTrack(get("HSPA8", org.Hs.egSYMBOL2EG), TxDb.Hsapiens.UCSC.hg19.knownGene)[[1]]
optSty <- optimizeStyle(trackList(repA, lollipopData, gene, heightDist = c(3, 3, 1)), theme="col")
trackList <- optSty$tracks
viewerStyle <- optSty$style
gr <- GRanges("chr11", IRanges(122929275, 122930122))
setTrackStyleParam(trackList[[2]], "ylabgp", list(cex=.8))
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)
addGuideLine(122929538, vp=vp)
## plot with customized geneTrack
dat <- gene$dat
mcols(dat) <- NULL
dat <- subsetByOverlaps(dat, gr)
dat$feature <- 'exon' # feature is required
dat$featureID <- paste0('name', seq_along(dat)) # treat each as single exon gene
dat$color <- sample(seq.int(7), length(dat), replace = TRUE) # set the color
dat$height <- sample(c(0.5, 1, 2), length(dat), replace = TRUE) # set the height
dat$hide_label <- TRUE # do not add labels to the block
gene <- new('track', dat=dat, dat2=SNPs, type='gene', name='a name')
optSty <- optimizeStyle(trackList(fox2, gene), theme="col")
trackList <- optSty$tracks
viewerStyle <- optSty$style
vp <- viewTracks(trackList, gr=gr, viewerStyle=viewerStyle)

## ----sessionInfo, results='asis'----------------------------------------------
sessionInfo()

