# Report breakdown by barcode

# Description

Read distribution plot - plot shows number of reads assigned during read grouping
Filtered Reads - plot shows percentage of assigned reads that have been recognized as PRIMER DIMERS or filtered based on low alignment score
Edit rates - plot gives overview of percentage of reads (not filtered as PRIMER DIMER) that have edits
Frameshift - plot shows what percentage of reads that have frameshift
Read heterogeneity plot - shows what is the share of each of the unique reads in total count of all reads. The more yellow each row, the less heterogeneity in the reads, more black means reads don’t repeat often and are unique
Top unassigned reads - take a look at the alignment of most abundant forward and reverse complemented reverse reads for each barcode, if you find that there is many unassigned reads you can ivestigate here.

# Barcode Summary

## Groups IDs

group | IDs
--- | ---
barcode_1 | ID_1, ID_2
barcode_2 | ID_3, ID_4, ID_5

## Read distribution

## Filtered Reads

## Edit rates

## Frameshift

## Heterogeneity of reads

# Top unassigned reads

## barcode_1

Forward | Reverse | Counts | Frequency
--- | --- | --- | ---
P1 | S1 | 1 | 0.0588235
P2 | S2 | 1 | 0.0588235
P3 | S3 | 1 | 0.0588235
P4 | S4 | 1 | 0.0588235

    P1                 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTGTTTTGATGATAAA     50
                         ||   ||| |    | | |||   |   ||    |||          |||
    S1                 1 AAG--CTGAC----GGCTAAA---TGAAAAATATCTG----------AAA     31

    P1                51 CTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTT--GGCAGAG-----G     93
                         | |       || ||  ||  ||||  | | ||    |||||||     |
    S1                32 CAT-------CTGTTCCAG--GTGCTGCGTATGCCAGGGCAGAGAAGAAG     72

    P1                94 GTCAGC--AGACCAGT--AAGTCTTCTCAATTTCTTTTATTT----ATGT    135
                         |||||   ||  || |  | |||      ||  | ||| ||     |
    S1                73 GTCAGGGAAGGTCACTGGAGGTCACTGGGATACCCTTTCTTCCCACACCA    122

    P1               136 AT-----ATGTAGT-------GATAAA-A    151
                         ||     | | |||       ||| |  |
    S1               123 ATGGGGAAAGGAGTCCTGCCAGATGACCA    151

    P2                 1 AAATACTGTCTTGTGACCAAACCTTCTTAAGGTGCTG---TTTTGATGAT     47
                                 | || | || | |  | | |||      |   ||  || ||
    S2                 1 --------TTTTATCACTACATATACATAAATAAAAGAAATTGAGAAGAC     42

    P2                48 NNNCTTTATTGTGCTTTTGTAGTTGTGCCCCTTGTGTTGGCAGAGGGTCA     97
                              ||| ||  ||  ||    | ||||         |||| |    ||
    S2                43 -----TTACTGGTCTGCTGACCCTCTGCCAACACAAGGGGCACAACTACA     87

    P2                98 GCAGACCAGTAAG-----TCTTCTCAATTTCTTTTATTTATGTATATGTA    142
                           ||  || |||      || ||  ||   |   |   || | |  | |
    S2                88 AAAGCACAATAAAGNNNATCATCAAAACAGCACCT---TAAGAAGGTTTG    134

    P2               143 GTGATAAAA--------    151
                         || | || |
    S2               135 GTCACAAGACAGTATTT    151

    P3                 1 AAGCTGACGGCTAAATNNNAAATATCTGAAACATCTGTTCCAGGTGCTGC     50
                         ||||||||||||||||   |||||||||||||||||||||||||||||||
    S3                 1 AAGCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50

    P3                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S3                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100

    P3               101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC    150
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S3               101 GATACCCTTTCTTCCCACACCAATGGGGAAAGGAGTCCTGCCAGATGACC    150

    P3               151 A    151
                         |
    S3               151 A    151

    P4                 1 AAGCTNGCGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50
                            ||  |||||||||||||||||||||||||||||||||||||||||||
    S4                 1 NNNCTGACGGCTAAATGAAAAATATCTGAAACATCTGTTCCAGGTGCTGC     50

    P4                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100
                         ||||||||||||||||||||||||||||||||||||||||||||||||||
    S4                51 GTATGCCAGGGCAGAGAAGAAGGTCAGGGAAGGTCACTGGAGGTCACTGG    100

    P4               101 GATACCCTTTCTTCCCACACCANNNGGGAAAGGAGTCCTGCCAGATGACC    150
                         |||||||   ||||||||||||   ||||||||||||||    |||||||
    S4               101 GATACCCNNNCTTCCCACACCAATGGGGAAAGGAGTCCTNNNNGATGACC    150

    P4               151 A    151
                         |
    S4               151 A    151